Monkeypox-virus (MPXV)

Antibody Information

Name	Monkeypox virus (MPXV) antibody
Catalog Number	R386t7	R387c6	R392a3	R396t5	R395a6	R399e2	R401c8	R400e7	R417t6	R418t3
Immunogen	Recombinant MPXV A35R antigen			Recombinant MPXV A29L antigen			Recombinant MPXV B6R antigen		Recombinant MPXV M1R antigen
Category	Fully human monoclonal antibody
Reactive Species	Monkeypox virus
Application Platform & Recommended Pairs	Immunochromatography (ICA)
	R386t7 (Capture) - R392a3 (Detection) R387c6 (Capture) - R392a3 (Detection)			R396t5 (Capture) - R399e2 (Detection) R396t5 (Capture) - R395a6 (Detection)			R401c8 (Capture) - R400e7 (Detection)		R417t6 (Capture) - R418t3 (Detection)
Purity	Protein A/G purified, purity >95%
Buffer	1xPBS, pH 7.4
Storage	Store at -20°C or below, aliquot to avoid repeated freeze-thaw cycles.

Antigen Information

Name	Monkeypox virus (MPXV) antigen
Catalog Numbers	C1618	C1619	C1620	C1621	C1622	C1624	C1625
Gene Name	A29L		A35R		B6R	M1R
Species	Monkeypox Virus
Type	Recombinant
Tag	His
Expression System	Eukaryotic	Prokaryotic	Eukaryotic	Prokaryotic	Eukaryotic	Eukaryotic	Prokaryotic
Uniprot ID	Q9YN60		Q80KX2		Q773E2	Q80KX3
Predicted MW	13.3 kDa (including tag)		14.3 kDa (including tag)		31.6 kDa (including tag)	20.4 kDa (including tag)
SDS-PAGE	The predicted molecular weights of A29L, A35R, B6R, M1R are 13.3 kDa, 14.3 kDa, 31.6 kDa, 20.4 kDa respectively.
Purity	>90%, assessed by R250-stained SDS-PAGE
Buffer	1x PBS, pH 7.4
Storage	Store at -80°C, aliquot to avoid repeated freeze-thaw cycles

Product Data

A35R Antibody Bioactivity Verification

Using ELISA, the Monkeypox virus A35R antigen (C1620, C1621) was used to detect MPXV A35R monoclonal antibodies R383e5, R387c6, R386t7, R384c2, and R392a3. The EC50 values for R383e5 and R387c6 were 7.29 ng/mL and 9.86 ng/mL, respectively.

Figure 1: ELISA results for A35R antibody (R383e5)

Figure 2: ELISA results for A35R antibody (R387c6)

A29L Antibody Bioactivity Verification

Using ELISA, the Monkeypox virus A29L antigen (C1619) was used to detect MPXV A29L monoclonal antibody R395a6. The EC50 was 10.54 ng/mL.

Figure 3: ELISA results for A29L antibody (R395a6)

B6R Antibody Bioactivity Verification

Using ELISA, the Monkeypox virus B6R antigen (C1622) was used to detect MPXV B6R monoclonal antibody R401c8. The EC50 was 15.26 ng/mL.

Figure 4: ELISA results for B6R antibody (R401c8)

A35R Antibody Colloidal Gold Platform Verification

Colloidal gold strips were prepared using the Monkeypox virus A35R antibody. The strips were coated with A35R antibodies R386t7, R387c6, and R384c2, and labeled with A35R antibody R392a3. The A35R antigen (C1620) was detected at concentrations of 0, 0.01, 0.1, 1, 10, and 100 ng/mL. The T line color showed a clear gradient change, with all three antibody pairs having a detection sensitivity of 10 pg/mL.

Figure 5: Colloidal gold detection results for Monkeypox virus A35R antibody

A29L Antibody Colloidal Gold Platform Verification

Colloidal gold strips were prepared using the Monkeypox virus A29L antibody. The strips were coated with A29L antibody R396t5 and labeled with A29L antibodies R399e2 and R397e8. The A29L antigen (C1619) was detected at concentrations of 0, 0.01, 0.1, 1, 10, and 100 ng/mL. The T line color showed a clear gradient change, with both antibody pairs having a detection sensitivity of 10 pg/mL.

Figure 6: Colloidal gold detection results for Monkeypox virus A29L antibody

B6R Antibody Colloidal Gold Platform Verification

Colloidal gold strips were prepared using the Monkeypox virus B6R antibody. The strips were coated with B6R antibody R401c8 and labeled with B6R antibody R400e7. The B6R antigen (C1622) was detected at concentrations of 0, 0.01, 0.1, 1, 10, and 100 ng/mL. The T line color showed a clear gradient change, with a detection sensitivity of 10 pg/mL for both antibodies.

Figure 7: Colloidal gold detection results for Monkeypox virus B6R antibody

M1R Antibody Colloidal Gold Platform Verification

Colloidal gold strips were prepared using the Monkeypox virus M1R antibody. The strips were coated with M1R antibody R417t6 and labeled with M1R antibody R418t3. The M1R antigen (C1624) was detected at concentrations of 0, 0.0125, 0.05, 0.1, and 1 ng/mL. The T line color showed a clear gradient change, with a detection sensitivity of 10 pg/mL for both antibodies.

Figure 8: Colloidal gold detection results for Monkeypox virus M1R antibody

Project Introduction

Monkeypox virus (MPXV) is an enveloped double-stranded DNA virus belonging to the Orthopoxvirus genus. MPXV is approximately 200x250 nm in size, with an ovoid or brick-shaped structure. The core is surrounded by a double-layer lipid membrane, which has randomly arranged tubular elements on its surface. MPXV can effectively infect primary human monocytes. Unlike Cowpox virus (CPV), MPXV does not interfere with MHC expression or intracellular transport to influence immune responses. Instead, it suppresses CD4+ and CD8+ T cell activation, eliminates local T cell responses, and avoids systemic immunosuppression and immune surveillance.
The MPXV genome is a linear double-stranded DNA molecule (about 197 kb) with inverted terminal repeats (ITRs) at the ends. The ITRs contain a set of short tandem repeat sequences and multiple open reading frames (ORFs). The central region of the genome encodes housekeeping genes, while the terminal variable regions encode virulence and host range genes. MPXV has a low dependency on host cell DNA and RNA replication, and it replicates intracellular mature viruses (IMV) and extracellular enveloped viruses (EEV) in the cytoplasm of the host cell. IMVs have a robust physical structure for inter-host transmission, while the more fragile EEVs are enveloped, limiting host immune clearance and facilitating intercellular virus spread.

Reference

1.Sarah Keasey, Christine Pugh, Alexander Tikhonov, et al. "Proteomic Basis of the Antibody Response to Monkeypox Virus Infection Examined in Cynomolgus Macaques and a Comparison to Human Smallpox Vaccination." Plos One, 2010, 5(12): e15547.
2.Emmanuel Alakunle, Ugo Moens, Godwin Nchind, et al. "Monkeypox Virus in Nigeria: Infection Biology, Epidemiology, and Evolution." Viruses, 2020, 12(11): 1257.
3.S. N. Shchelkunov, A. V. Totmenin, P. F. Safronov, et al. "Analysis of the Monkeypox Virus Genome." Virology, 2002, 297: 172-194.